HuProt™人全蛋白质组芯片

　　HuProt™人全蛋白质组芯片是目前容量最大的人类蛋白质芯片，在一张玻片上包含了数量最多的全长人类蛋白（大于21000个）。芯片上的人类全长蛋白从真核模式生物酵母表达系统中纯化而来，保持了其天然构象和翻译后修饰如磷酸化、糖基化等，每个表达蛋白均含GST标签，可用Anti-GST antibody进行质控检测。

•       目前容量最大（大于21000个）

        基本涵盖全部人类蛋白

•       蛋白均为真核生物酵母表达

        保持一定的天然构象和翻译后修饰

•       应用广泛

        能应用于所有与蛋白互作的研究中

•       高性价比

        提供组学数据，让您整体把握研究方向

        有效缩小您的研究焦点，提高效率

部分发表文章

Autoantibody Profiling

• Fan W et al. (2019) Sa1679–Multiple rather than specific Antibodies were identified in Irritable Bowel Syndrome using Huprot™ Microarray Approach Gastroenterology 156 (6), Supplement 1, Page S-364
• Lastwika KJ et al. (2019) Tumor-derived autoantibodies identify malignant pulmonary nodules. Am J Respir Crit Care Med, 199 (10)
• Ye S et al. (2019) Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China  Clin Proteomics,16:22
• Gowen MF et al. (2018) Baseline antibody profiles predict toxicity in melanoma patients treated with immune checkpoint inhibitors. J. Transl. Med., 16(1):82

Antibody Specificity and Crossreactivity

• Venkataraman A et al. (2018) A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods , in press
• Ramos-López et al. (2018) Antibody Specificity Profiling using Protein Microarrays. In: Rockberg J., Nilvebrant J. (eds) Epitope Mapping Protocols, Methods in Molecular Biology, vol 1785. e0181251.

Protein Interactomics

• Zhao T et al. (2019) DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma  Theranostics; 9(21): 6175–6190
• Song G et al. (2019) Proteome-wide Tyrosine Phosphorylation Analysis Reveals Dysregulated Signaling Pathways in Ovarian Tumors. Mol Cell Proteomics. 18(3):448-460.
• Cao T et al. (2019) A Two-Way Proteome Microarray Strategy to Identify Novel Mycobacterium tuberculosis-Human Interactors. Front. Cell. Infect. Microbiol, 28;9:65
• Cho HM et al. (2019) Drp1-Zip1 Interaction Regulates Mitochondrial Quality Surveillance System Mol Cell. 73(2):364-376.e8

Kinase assays

• Hu J et al. (2015) Systematic prediction of scaffold proteins reveals new design principles in scaffold-mediated signal transduction. PLoS Comput Biol 11(9):e1004508
• Tarrant MK et al. (2012) Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis. Nat Chem Biol 8(3):262-9.

Other enzyme assays

• Uzoma I et al. (2018) Global Identification of Small Ubiquitin-related Modifier (SUMO) Substrates Reveals Crosstalk between SUMOylation and Phosphorylation Promotes Cell Migration.  Mol. Cell. Proteomics 17(5):871-888
• Xu Z et al. (2017) Systematic identification of the protein substrates of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T1/T2/T3 using a human proteome microarray.  Proteomics 17:11

 Nucleic Acid Binding

• Kang CL et al. (2019) LncRNA AY promotes hepatocellular carcinoma metastasis by stimulating ITGAV transcription Theranostics 9(15):4421-4436
• Liu L et al. (2019) Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome. Nucleic Acid Res. 47(5):2244-2262
• McClymont et al. (2018) Parkinson-Associated SNCA Enhancer Variants Revealed by Open Chromatin in Mouse Dopamine Neurons. Am J Hum Genet 103(6):874-892

Small molecule profiling

• Jia D et al (2019) Cardioprotective mechanism study of salvianic acid A sodium based on a proteome microarray approach and metabolomic profiling of rat serum Mol. Omics 2019 15(4):271-279.
• Zhang HN et al (2015) Systematic identification of arsenic-binding proteins reveals that hexokinase-2 is inhibited by arsenic. Proc Natl Acad Sci U.S.A. 112 (49):15084-9

Other

• Syu GD (2019) Development and application of a high-content virion display human GPCR array. Nature Communications 10: 1997
• Coll JM (2018) Herpesvirus infection induces both specific and heterologous antiviral antibodies in carp. Front Immunol. 9 (39) Jan 2018